Application of Jeevamrit Improves Soil Properties in Zero Budget Natural Farming Fields

Zero Budget Natural Farming (ZBNF), utilizing natural resources, multiple cropping systems, and cow-dung- urine-based products to improve soil biology, has been practiced by thousands of farmers in India. However, without any scientific proof, this traditional ancient technique is mocked as a bugged theory the community. In current study, we have investigated effect Jeevamrit—cow-dung- formulation—on chemical microbial properties ZBNF field coupled with metagenomic analysis economics ZBNF. The percentage increase properties, such organic carbon, available phosphorus, potassium, was recorded up 46%, 439%, 142%, respectively, while micronutrients, Zn, Fe, Cu, Mn, also increased 98%, 23%, 62%, 55%, from 2017 2019. Whole genome revealed that Proteobacteria were dominantly present, bacterial phyla including Bacillus, Pseudomonas, Rhizobium, Panibacillus. On other hand, Ascomycota dominating fungal present sample. Further, functional showed high representation genes/enzymes involved amino acids carbohydrate metabolism contributing fertility, plant growth, defense, development. Additionally, cost–benefit ratio double farmer’s practice when tested rice wheat system. results study provide new proof concept understanding potential component, i.e., Jeevamrit, improving properties.